Table 2. Residues in clones from subject ACH18839 with positively charged side chains substituted for residues with neutral, negatively charged chains.

	V1/V2			C3			V4					V5	2G12 IC50
AAposition	151	160	187	336	343	347	407	408	409	410	412	463
R4.15F10	__	E**	G	K*	R*	D**	T	L	L	__	__	G	< 0.20
R4.43A11	K*	N	N	E**	K*	V	N	N	T	E**	E**	A	2.45
R4.15H6	K*	M	D**	E**	K*	N	N	T	T	E**	G	E**	> 12.50
R4.15F9	K*	M	D**	V	K*	A	N	T	T	E**	E**	E**	> 12.50
R4.15D5	G	N	D**	T	Q	K*	__	T	T	E**	__	S	> 12.50
R4.15A9	K*	M	D**	E**	K*	N	N	T	T	E**	G	E**	> 12.50
R4.43E1	K*	N	N	E**	K*	V	N	N	T	E**	E**	A	> 12.50
R4.43B12	K*	N	N	E**	K*	V	N	N	T	E**	E**	A	> 12.50

Regions of Envelope are noted in the top bar and below are the amino acid (AA) positions. Each row is an Env clone. * Positively and ** negatively charged amino acids are in bold. 2G12 IC₅₀ (µg/mL) is specified for each clone.