Figure 3. NLHS increased Hsp70 in L. vannamei post-larvae.

Protein extracts were resolved by electrophoresis in SDS polyacrylamide gels and either stained with Coomassie Biosafe (A) or blotted to polyvinylidene fluoride membranes and incubated with antibody to Hsp70 (B). Approximately 50 µg of protein was loaded in each lane. 28, non-heat shocked post-larvae heat shock at 30°C, 32°C, 34°C, 36°C and 38°C was for 30 min with recovery at 28°C for 8 h H-Hsp70: Human Hsp70 recombinant protein M: molecular mass standards in kDa. The figure is a representation from two separate experiments.