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. 2013 Sep 9;8(9):e73199. doi: 10.1371/journal.pone.0073199

Figure 4. NLHS enhanced Hsp70 production in L. vannamei.

Figure 4

Post-larvae were exposed to 30 min heat shock from 28°C to 30°C, 32°C, 34°C, 36°C and 38°C, then transferred to 28°C for 8 h. Quantification of Hsp70 was by ELISA. A standard curve, constructed with Human Hsp70 recombinant protein was used to convert ELISA readings obtained with shrimp tissue protein extracts to Hsp70 content. Bars represent the fold difference of Hsp70 quantity in comparison to a non-heated control. The error bars represent the SD from 3 replicates. Asterisks denote statistically significant differences between values obtained for control and heat shocked post-larvae (P<0.05). The figure is a representation from two separate experiments.