Figure 2. Decreased 3D Tube Formation by PAECs from PPHN fetal Sheep after ET-1 treatment.
Growth and tube formation were assessed in PPHN fetal PAECs with and without ET-1 treatment. ET-1 treatment had no effect on PPHN PAEC growth. Tube formation by PPHN PAECs was significantly decreased when compared with age-matched controls. Tube length was decreased by 27% (p<0.01) and the number of branch points/HPF was decreased by 32% (p<0.01) in PPHN PAECs. ET-1 treatment (100nM) further decreased tube length 24% (p<0.05) and number of branch points/HPF by 28% (p<0.05) in PPHN fetal PAECs.