TABLE 1.
Primary and second antibodies employed
| Antibodies | Vectors | Identification | Dilution | Source | Specificity |
|---|---|---|---|---|---|
| GSTα4 | Santa Cruz Biotechnology, Santa Cruz, CA | Cat# sc-241483 | 1:50 (dilution with 1 % BSA–PBS) | Goat polyclonal antibody | Reacts with human, mouse, bovine, and porcine |
| F4/80 | San Diego, CA | Cat# 14-4801-85 | 1:50 (dilution with 1 % BSA–PBS) | Rat monoclonal antibody | Reacts with mouse |
| Desmin | Abcam, Cambridge, MA | Cat# Ab32362 | 1:100 (dilution with 1 % BSA–PBS) | Rabbit monoclonal antibody | Reacts with mouse, rat, guinea pig, and human |
| Collagen IV | Abcam, Cambridge, MA | Cat# Ab6586 | 1:100 (dilution with 1 % BSA–PBS) | Rabbit polyclonal antibody | Reacts with mouse, rat, cow, human, and pig |
| Isolectin GS-IB4-conjugated Alexa Fluor 568 conjugate | Invitrogen, Eugene, OR | Cat# 121411 | 1:100 (dilution with 1 % BSA–PBS) | Griffonia simplicifolia, | Reacts with mouse and bovine |
| Alexa Fluor 488-conjugated goat anti-rabbit IgG | Invitrogen, Eugene, OR | Cat# A-11008 | 1:100 (dilution with 1 % BSA–PBS) | Goat | Reacts with rabbit |
| Alexa Fluor 647-conjugated goat anti-rabbit IgG | Invitrogen, Eugene, OR | Cat# A-21245 | 1:100 (dilution with 1 % BSA–PBS) | Goat | Reacts with rabbit |
| Alexa Fluor 488-conjugated goat anti-rat IgG | Invitrogen, Eugene, OR | Cat# A-11006 | 1:100 (dilution with 1 % BSA–PBS) | Goat | Reacts with rat |
| Alexa Fluor 647-conjugated goat anti-rat IgG | Invitrogen, Eugene, OR | Cat# A-21247 | 1:100 (dilution with 1 % BSA–PBS) | Goat | Reacts with rat |
| Alexa Fluor 488-conjugated rabbit anti-rat IgG | Invitrogen, Eugene, OR | Cat# A-21210 | 1:100 (dilution with 1 % BSA–PBS) | Rabbit | Reacts with rat |
| Alexa Fluor 568-conjugated rabbit anti-goat IgG | Invitrogen, Eugene, OR | Cat# A-11079 | 1:100 (dilution with 1 % BSA–PBS) | Rabbit | Reacts with goat |