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. 2013 Jul 3;14(5):645–659. doi: 10.1007/s10162-013-0404-1

FIG. 7.

FIG. 7

Excess Bcl2 protects hair cells from gentamicin damage. Overexpression of hair cell-specific Bcl2 does not protect hair cells from A acute neomycin (two-way ANOVA, F(1, 100) = 0.65, P = 0.42). Significant protection is seen from B acute gentamicin toxicity (two-way ANOVA, F(1, 73) = 113.5, P < 0.001) or C continuous gentamicin exposure (two-way ANOVA, F(1, 90) = 181.60, P < 0.001). Data represent counts of antiparvalbumin-labeled hair cells from seven neuromasts per fish, N = 7–19 fish per group, data are presented as mean ± 1 SD. Asterisks represent significant pairwise differences using Bonferroni-corrected post hoc testing (**P < 0.01, ***P < 0.001). D Confocal images (z-series projections) of *AB (left column) and Tg(myo6b:EGFP-bcl2) (right column) neuromasts labeled with antiparvalbumin (red) and anti-GFP (green). Images are shown of control neuromasts (top row) and those treated with 100 μM gentamicin for 6 h (bottom row), demonstrating robust hair cell survival in gentamicin-treated transgenic hair cells. The scale bar in the upper left panel is 5 μM and applies to all panels.