Table 1.
Novel protein associations and corresponding developmental regulatory mechanisms identified by affinity purification-mass spectrometry (AP-MS).
| Organism | Bait | Interactor(s) | Tag | Source cell type | Validated function | References |
|---|---|---|---|---|---|---|
| Worm | MIS-12, KBP-1 | 10 kinetochore proteins | LAP (GFP-S) | Transgenic worms | Outer kinetochore assembly, chromosome segregation | 40 |
| Worm | DCR-1 | Several associated proteins | 8xHA | Transgenic worms | Role of DCR-1 in multiple small-RNA-mediated pathways | 41 |
| Fly | Deltex (Dx) | Kurtz (Krz) | TAP (ProtA-CBP) | Stable Kc167 cell line | Downregulation of Notch signaling | 42 |
| Fly | Archipelago (Ago/Fbxw7) | Myc (dm) | TAP (ProtA-CBP) | Stable Kc167 cell line | Degradation of Myc, control of cell growth | 43 |
| Fly | Myopic (Mop/PTPN23) | Yorkie (Yki) | GS-TAP (ProtG-SBP) | Stable S2 cell line | Endocytic regulation of Yorkie/Hippo signaling | 38 |
| Zebrafish | Elipsa | Intraflagellartransport (IFT) particle proteins | TAP (ProtA-CBP) | Transfected zebrafish embryos | Regulation of intraflagellartransport andciliogenesis | 44 |
| Mouse | Cyclin D1 | Transcription factors | FLAG-HA | Mouse organs from tagged knock-in lines | Transcriptional function of Cyclin D1 in development | 45 |
| Mouse | Oct4 (POU5F1) | Several associated proteins | 3xFLAG and 3xFLAG-CBP | Embryonic stem cell lines | Expanded view of Oct4 regulatory network and pluripotent cell identity | 46, 47 |
| Human | Disheveled (Dvl) | KLHL12, Cullin-3 (CUL3) | SBP-HA-CBP | Stable HEK293T cell line | Degradation of Dsh, downregulation of Wnt signaling | 48 |
| Human | WTX (FAM123B) | β-catenin, other proteins | SBP-HA-CBP | Stable HEK293T cell line | Degradation of β-catenin, downregulation of Wnt signaling | 49 |