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. 2013 Sep 10;3:2601. doi: 10.1038/srep02601

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(a) pH change of the medium by native urease, Au nanoparticle-urease composite (urease reduced Au nanoparticles), denatured urease and functionalized Au nanoparticles with urease (b) Emission spectra of ANS in native urease, Citrate capped Au nanoparticles-urease composite and Au nanoparticle-urease composite (urease reduced Au nanoparticles) depicting the conformational modifications in enzyme structure (λ_ex = 370 nm). (c) Circular dichroism spectrum of native urease and Au nanoparticle urease composite in water. (d) UV-visible spectrum of DTNB treated urease after incubation with HAuCl₄ for 48 hours, showing the absence of SPR band of Au nanoparticles.