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Determination of Km for nitroso substrates against E. coli DXP synthase (DXPS) compared to Porcine PDH E1 subunit
| Substrate | PDH Km, μM[a] |
KmPDH,/KmDXPS |
|---|---|---|
| 1 | 350 ± 30 | 2.7 |
| 4 | 408 ± 60 | 7.5 |
| 6 | 450 ± 16 | 19.3 |
Performed in triplicate. Values shown are the average ± SEM.
Reaction conditions: 100 mM HEPES, pH 8.0, 2 mM MgCl2, 5 mM NaCl, 1 mM ThDP, 1 mg/mL BSA, 10 – 20 mM pyruvate, 10% DMSO v/v, 37 °C
