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. Author manuscript; available in PMC: 2013 Sep 10.
Published in final edited form as: J Immunol. 2009 Feb 1;182(3):1379–1385. doi: 10.4049/jimmunol.182.3.1379

FIGURE 3.

FIGURE 3

Fixed mature BM-DC function in the anti-Tim-1 mAb proliferation assay. Naive CD4+CD25− T cells purified from C57BL/6 mice were cultured with immature or mature MHC class II-deficient DC, in the presence of increasing dose of anti-Tim-1 mAb. Immature and mature MHC class II-deficient DC were fixed with 2% paraformaldehyde in PBS prior addition to the culture. Tritiated thymidine incorporation (cpm) was measured after 48 h of culture. Bars represent the mean ± SEM of one of three consecutive experiments yielding similar results.