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. Author manuscript; available in PMC: 2013 Sep 10.
Published in final edited form as: J Immunol. 2009 Feb 1;182(3):1379–1385. doi: 10.4049/jimmunol.182.3.1379

FIGURE 5.

FIGURE 5

In vivo stimulation of Tim-1 induces polyclonal T cell proliferation. CFSE-labeled splenocytes from C57BL/6 mice were injected into irradiated allogeneic DBA/2 hosts treated with agonist anti-Tim-1 mAb or IgG2a control mAb (250 μg/ml). Three days later, proliferation was assessed by analysis of the CFSE profile of CFSE-labeled CD4+ T cells (A). Proliferative T cells that are programmed for apoptosis were identified by flow cytometry as the annexin V+ CFSE-labeled CD4+ T cells (B). Data are representative of four consecutive experiments yielding similar results.