Fig. 2.

Spatial reorganization of retinal neurons and RPE cells in coculture. Retinal neurons were seeded first and ARPE-19 cells were seeded on top of them 1 day later. Photomicrographs show the cocultures at 0 (A), and after 2 hr (B) and 5 hr (C). The cartoon in (D) represents the relative spatial arrangement of photoreceptors and RPE cells at the end of the reorganization process. Note that after 2 and 5 hr, lamellipodia edges (white arrows in B,C) grew and extended until they reached neuronal cell bodies (black arrows in B,C) or axons (arrowheads in B,C). RPE cells ended beneath neurons (C,D), resembling the relative spatial arrangement found in vivo. Note that the axons that were oriented toward the epithelial cells underwent a rapid retraction (compare white arrowheads in B,C). Scale bars = 10 µm.