TABLE I.

Spatial Reorganization of Retinal Neurons and RPE Cells in Co-Culture

	Neurons over RPE cells/dish					Photoreceptors over RPE cells/dish
	Number			(%)		Number			(%)
Time (hrs)	Mean	SD		Mean	SD	Mean	SD		Mean	SD
0	0.0 ± 0.0		a	0.0 ± 0.0		0.0 ± 0.0		a	00.0 ± 0.00
1	06621.0 ± 13137.0		a	06.3 ± 12.6		06016.0 ± 11931.1		a	06.3 ± 11.7
2	30267.3 ± 5464.50		b	28.8 ± 6.30		28312.6 ± 8244.70		b	31.6 ± 8.40
5	74110.9 ± 10634.4		c	70.5 ± 10.1		71420.5 ± 10763.7		c	81.2 ± 18.2
24	90715.4 ± 10535.3		c	86.3 ± 10.0		88004.0 ± 11793.2		c	98.2 ± 2.50

Retinal neurons were seeded on the plastic dishes and RPE cells were seeded over them 1 day later. The data represent the amount of neurons and photoreceptors, respectively, found on top of RPE cells at different times in co-culture. The values were calculated from the total number of cells found in the close proximity of RPE cells (i.e., less than 100 lm from each other). Data are the mean ± SD of 5 experiments. Statistical significance was calculated by using One-Way Repeated Measures ANOVA test for each column. At the 0.05 level, the population means are significantly different. Mean values were compared using Bonferroni t statistics. Letter (a–c) indicate significant differences ( p < 0.05) among the cases analyzed.