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Localization of the V-ATPase B1 and a4 subunit isoforms in epididymal clear cells
The distal region (cauda) of rat epididymis was immunofluorescently labeled with antibodies against a4 (A and D: green) and B1 (B and E: red). C and F: merged images from A and B, and D and E, respectively. B1 and a4 colocalize in the apical membrane and subapical vesicles in clear cells exclusively. Nuclei and sperm are labeled in blue with DAPI. Bars = 50 μm (A, B, C) and 5 μm (D, E, F).
