Table 1.
Detection of rAd and wtAd Sequences in Cellular DNA from Liver and Colon Tissue of OTC-Deficient Heterozygote Patients Who Received AdOTC Gene Therapy Trial More Than a Decade Ago
| |
|
|
|
Patient 1 (liver cancer) |
Patient 2 (colon cancer) |
|
|
|---|---|---|---|---|---|---|---|
| Sequence for amplification | Target for detection | Template for validation | PCR sensitivity (copy/reaction) | Normal tissue (10 samples) | Tumor tissue (12 samples) | Tumor tissue (1 sample) | Sequencing |
| Ad DNA polymerase gene | rAd and wtAd | rH5.001CB-hOTC vector DNA and wtAd5 plasmid DNA | 1 | — | — | — | Verified |
| hOTC transgene/remnant E1 gene junction | rAd | rH5.001CB-hOTC vector DNA | 1 | — | — | — | Verified |
| Native Ad E1 sequence | wtAd | wtAd5 plasmid DNA | 1 | —a | —a | — | Verified |
a
Two samples tested.