Figure 1. Expression and Purification of rNS3 (a), rRGD-NS3 (b), rNT-GP96 (c) and rCT-GP96 (d) in E. coli M15 Strain on SDS-PAGE 12.5%. Protein MW Marker in kDa (MW), Bacterial Extract Before Induction by IPTG (Lane 2), Crude Bacterial Lysate 4 h After Induction (Lane 3), Recombinant Proteins Purified From Bacterial Lysate by Affinity Chromatography Using Ni-NTA Resin (Lane 4) and Western Blot Assay of Recombinant Protein With His-Tag Monoclonal Antibody (lane 5).