Figure 3. Expression of IL-12 (A), TNFα (B) and α5integrin (C) in THP-1 Macrophages Treated With Recombinant Proteins. The Cells Were Cultured for 24 h With rNS3 (3µM), rRGD-NS3 (3µM) Alone or in Combination With rGP96 Fragments (2.5µM) and LPS (1 µg/mL). The mRNA Expression Was Measured by Real-time PCR and Analyzed With the 2 - ΔΔCT Method. Comparisons Between Treated and Control Cells Were Performed Using a One-way ANOVA.

a P < 0.05, b P < 0.01 and c P < 0.001