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Effect of inoculum size on polygalacturonase yield. Inoculum density was varied from 2.5 mL to 15 mL (2.5 × 104 CFU/mL). The other culture conditions were particle size of wheat bran: 500-1000 μm; moisture: 70%; pH of the mineral salt solution: 6.0; and temperature: 30°C.
