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Time course of polygalacturonase production. Enzyme yield was monitored at regular intervals. (■) Enzyme activity (U/g), (♦) Biomass (glucosamine (mg/g)). The culture conditions were particle size of wheat bran: 500-1000 μm; moisture: 70%; pH of the mineral salt solution: 6.0; inoculum size 5 mL and temperature 30°C with 1% pectin as carbon supplement.
