Figure 1.

A. Enzymatic digests of the 3 clones (lanes1-3) of the BPV4 L1 capsid protein ORF DNA containing pMT/V5-His expression vector developed in an ethydium bromide-stained agarose gel. Molecular size is referenced to a GeneRulerTM 1kb DNA ladder (M); B. Total protein expression in the three BPV L1 ORF-expression vector transfected Drosophila S2 cells before (lanes 1-3) and after (lanes 4-6) mercaptoethanol reduction S2 non-transfected cells (lane 7) and molecular size references (M) in silver-stained polyacrylamide gel. C. Dot Blot analysis for BPV4 L1 capsid protein transcripts of transfected 1 and 2 (Co11 and Col2) and non-transfected (C-) S2 clones before and after β-mercaptoethanol reduction