Figure 1.

MPB64 gene-targeted PCR for detection of Mycobacterium tuberculosis. Electrophoretic separation of the amplicon into 2% agarose gel is documented across Lanes 1-7. Lanes 1-3 represent the clinical cerebrospinal fluid samples, Lanes 4 and 5 exemplify the colony-PCR from atypical Mycobacterium (Mycobacterium bovis) as negative control and lanes 6 and 7 stand for the colony-PCR from Mycobacterium tuberculosis culture of the clinical samples (other than CSF) as positive control. The presence of a 240 bp. amplicon in the Lanes 1, 2, 3, 6 and 7 indicated the presence of the target.