Table 1.

The primers used for detection of the various genes by PCR, the amplicon size and relevant references.

Gene	Primer	Oligonucleotide pairs (5´→3´)	Amplicon (bp)	Reference
LT	LTA-1	GGCGACAGATTATACCGTGC	696	Schultsz
	LTA-2	CCGAATTCTGTTATATATGTC		et al., 1994
STa	STI-1	TTAATAGCACCCGGTACAAGCAGG	147	Olsvik
	STI-2	CTTGACTCTTCAAAAGAGAAAATTAC		et al., 1993
STb	STb-1	ATCGCATTTCTTCTTGCATC	172	Blanco
	STb-2	GGGCGCCAAAGCATGCTCC		et al., 1997
VTl	VT1-A	GAAGAGTCCGTGGGATTACG	130	Pollard
	VT1-B	AGCGATGCAGCTATTAATAA		et al., 1990
VT2	VT2–3	CCGTCAGGACTGTCTGAAAC	726	Woodward
	VT2–5	GAGTCTGACAGGCAACTGTC		et al., 1992
pap	pap-1	GCAACAGCAACGCTGGTTGCATCAT	336	Yamamoto
	pap-2	AGAGAGAGCCACTCTTATACGGACA		et al., 1995
hly	hly-1	AACAAGGATAAGCACTGTTCTGGCT	1177	Yamamoto
	hly-2	ACCATATAAGCGGTCATTCCCGTCA		et al. 1995
iuc	iuc-1	TACCGGATTGTCATATGCAGACCGT	602	Yamamoto
	iuc-2	AATATCTTCCTCCAGTCCGGAGAAG		et al.,1995
cnf	cnf1	AAGATGGAGTTTCCTATGCAGGAG	498	Yamamoto
	cnf2	CATTCAGAGTCCTGCCCTCATTATT		et al.,1995
sfa	sfa-1	CTCCGGAGAACTGGGTGCATCTTAC	410	Yamamoto
	sfa-2	CGGAGGAGTAATTACAAACCTGGCA		et al. 1995
afa	afa-1	GCTGGGCAGCAAACTGATAACCTC	750	Yamamoto
	afa-2	CATCAAGCTGTTTGTTCGTCCGCCG		et al.1995