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A) line 1, PCR amplification of chit33 genomic DNA (approximately 1.2 Kb), line 2, digestion of recombinant plasmid containing chit33 gene, using XbaI, (two bands approximately 2.7 and 1.2 Kb), M= DNA size marker; B) line 1, PCR amplification of chit33 cDNA (approximately 1 Kb), line 2, digestion of PCR product using SacI , (two bands approximately o.6 and 0.4 Kb), M= DNA size marker
