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. 2008 Sep 1;39(3):433–437. doi: 10.1590/S1517-83822008000300005

Figure 4.

Figure 4

SDS – PAGE and molecular weight determination of Chit33 extracted from E. coli harboring chit33 gene. The gel was stained with Coomassie blue. Line 1, 2, 3, an 4- E. coli BL21 (DE3)- harboring pETSM2 induced with 0.2 mM IPTG for 2, 4, 6, and 8 hours, respectively. Line 5, 6, 7, an 8- E. coli BL21 (DE3)-harboring pET26b(+) induced with 0.2 mM IPTG for 8, 6, 4, and 2 hours, respectively. M- Protein molecular weight marker (kDa), arrows (a and b)- presence (a) or absence (b) of expressed protein after 4 hours induction with IPTG.