Table 1.
Name | Oligonucleotides Sequence |
MCH33f | 5′-GCTCTAGAATGCCTTCATTGACTGCTCTTGCG-3′ |
MCH33r | 5′-CGTCTAGATTACCTCAAAGCATTGACAACC-3′ |
CH33Pf | 5′-GGGTCTCGCATGCCTTCATTGACTGCTCTTGCG-3′ |
CH33Pr | 5′-CGAATTCTTACCTCAAAGCATTGACAACC-3′ |
M13F | 5′-GCTAGTTATTGCTCAGCGG-3′ |
M13R | 5′-GTAAAACGACGGCCAGT-3′ |
T7 promoter | 5’-TAATACGACTCACTATAG-3’ |
T7 terminator | 5’-CGATCAATAACGAGTCGC-3’ |