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Dot blot hybridization using the nifH probe and genomic DNA (0.1–3.0 mg) from A) COL; B) MANC; C) MAGDE3; D) MI753; E) E. coli (negative control); F) human DNA (negative control). In G: 0.1 mg of a plasmid containing A. brasilense Sp7 nifH gene (left spot) and 1mg of genomic DNA from Azospirillum brasilense Sp7 (right spot).
