Table 1.

Design matrix for the 2^8-3 fractional factorial design and the response after analysis.

Run	KH2PO4	(NH4)2SO4	KOH	MgSO4.7H2O	Fe(SO4)3.6H2O	Thiamine	Biotin	yeast cells	Protease Activity
	g/l				10-3 g/l			%	U/ml
1	19.6	3.0	6.4	0.3	2.0	2.0	2.0	1.5	0.27
2	19.6	3.0	6.4	0.3	0.0	2.0	2.0	0.5	0.10
3	19.6	3.0	6.4	0.1	2.0	2.0	0.0	0.5	0.10
4	19.6	3.0	6.4	0.1	0.0	2.0	0.0	1.5	0.23
5	19.6	3.0	2.0	0.3	2.0	0.0	2.0	0.5	0.06
6	19.6	3.0	2.0	0.3	0.0	0.0	2.0	1.5	0.15
7	19.6	3.0	2.0	0.1	2.0	0.0	0.0	1.5	0.21
8	19.6	3.0	2.0	0.1	0.0	0.0	0.0	0.5	0.07
9	19.6	1.0	6.4	0.3	2.0	0.0	0.0	0.5	0.07
10	19.6	1.0	6.4	0.3	0.0	0.0	0.0	1.5	0.24
11	19.6	1.0	6.4	0.1	2.0	0.0	2.0	1.5	0.20
12	19.6	1.0	6.4	0.1	0.0	0.0	2.0	0.5	0.09
13	19.6	1.0	2.0	0.3	2.0	2.0	0.0	1.5	0.12
14	19.6	1.0	2.0	0.3	0.0	2.0	0.0	0.5	0.09
15	19.6	1.0	2.0	0.1	2.0	2.0	2.0	0.5	0.08
16	19.6	1.0	2.0	0.1	0.0	2.0	2.0	1.5	0.15
17	7.6	3.0	6.4	0.3	2.0	0.0	0.0	1.5	0.54
18	7.6	3.0	6.4	0.3	0.0	0.0	0.0	0.5	0.19
19	7.6	3.0	6.4	0.1	2.0	0.0	2.0	0.5	0.28
20	7.6	3.0	6.4	0.1	0.0	0.0	2.0	1.5	0.55
21	7.6	3.0	2.0	0.3	2.0	2.0	0.0	0.5	.0.08
22	7.6	3.0	2.0	0.3	0.0	2.0	0.0	1.5	0.09
23	7.6	3.0	2.0	0.1	2.0	2.0	2.0	1.5	0.09
24	7.6	3.0	2.0	0.1	0.0	2.0	2.0	0.5	0.03
25	7.6	1.0	6.4	0.3	2.0	2.0	2.0	0.5	0.24
26	7.6	1.0	6.4	0.3	0.0	2.0	2.0	1.5	0.23
27	7.6	1.0	6.4	0.1	2.0	2.0	0.0	1.5	0.65
28	7.6	1.0	6.4	0.1	0.0	2.0	0.0	0.5	0.18
29	7.6	1.0	2.0	0.3	2.0	0.0	2.0	1.5	0.03
30	7.6	1.0	2.0	0.3	0.0	0.0	2.0	0.5	0.09
31	7.6	1.0	2.0	0.1	2.0	0.0	0.0	0.5	0.07
32	7.6	1.0	2.0	0.1	0.0	0.0	0.0	1.5	0.12
33*	13.6	2.0	4.2	0.2	1.0	1.0	1.0	1.0	0.19
34*	13.6	2.0	4.2	0.2	1.0	1.0	1.0	1.0	0.18
35*	13.6	2.0	4.2	0.2	1.0	1.0	1.0	1.0	0.23

^*center point