Table 4.

Results of the runs of the central composite design for optimization of the fermentation conditions.

Run	Temperature (°C)	Agitation (rpm)	Protease Activity (U/ml)
1	30	200	7.14
2	30	100	7.52
3	20	200	8.42
4	20	100	8.50
5	18	150	7.80
6	32	150	6.90
7	25	80	8.59
8	25	220	8.89
9*	25	150	8.55
10*	25	150	7.98
11*	25	150	8.31

^*center point.