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. 2009 Dec 1;40(4):725–733. doi: 10.1590/S1517-83822009000400002

Table 3.

Purification steps of extracellular chitinase from the strain ANU 6277

Purification step Total proteins (mg) Total activity(U) Specific activity (U.mG-1) Purification (fold)(%) Yield (%)
Culture filtrate 302 3570 11.8 0 100
(NH4)2 SO4 Precipitation 118 3120 26.4 2.2 87.4
Sephadex G-100 27.5 1649 59.9 5 46.2