Table 3.
Purification step | Total proteins (mg) | Total activity(U) | Specific activity (U.mG-1) | Purification (fold)(%) | Yield (%) |
---|---|---|---|---|---|
Culture filtrate | 302 | 3570 | 11.8 | 0 | 100 |
(NH4)2 SO4 Precipitation | 118 | 3120 | 26.4 | 2.2 | 87.4 |
Sephadex G-100 | 27.5 | 1649 | 59.9 | 5 | 46.2 |