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Estimation of the molecular mass of the phytate-degrading enzyme from Aspergillus niger by gel filtration.
The column was calibrated with A: glucose-6-phosphate dehydrogenase (Mr = 120,000), B: creatine kinase (Mr = 81,000), C: bovine serum albumin (Mr = 68,000), D: β-lactoglobulin (Mr = 40,000), and E: myoglobin (Mr = 17,000). P: phytate-degrading enzyme from Aspergillus niger (Mr estimated to be approximately 85,000). Phytate-degrading activity ( ■ ), optical density at 280 nm ( — )
