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Purification scheme for the phytate-degrading enzyme from Aspergillus niger
| purification step | total protein (μg) | total phytate- degrading activity (U) | Specific activity (U mg-1) | purification (fold) | recovery (%) |
|---|---|---|---|---|---|
| Crude extract | 54000 | 138 | 2.6 | - | - |
| 0-90% (NH4)2SO4 precipitation | 26539 | 117 | 4.4 | 1.7 | 84.8 |
| DEAE Sepharose CL6B | 5693 | 110 | 19.3 | 7.4 | 79.7 |
| CM Sepharose CL 6B | 1156 | 62 | 53.6 | 20.6 | 44.9 |
| Sephacryl S-200 HR | 287 | 36 | 125.4 | 48.2 | 26.1 |
| Mono S HR 5/5 | 211 | 28 | 132.7 | 51.0 | 20.3 |
