Table 1.
Effect of various parameters on L-asparaginase production by S.gulbargensis
| Parameter | L-asparaginase activity (IU) | |||||
|---|---|---|---|---|---|---|
| 24 h | 48 h | 72 h | 96 h | 120 h | 144 h | |
| pH | ||||||
| 6.5 | 3.0 | 3.9 | 4.8 | 5.7 | 6.4 | 6.0 |
| 7.0 | 3.2 | 4.2 | 5.5 | 6.6 | 7.3 | 6.8 |
| 7.5 | 3.5 | 5.2 | 6.5 | 7.3 | 8.1 | 7.2 |
| 8.0 | 3.9 | 6.6 | 7.8 | 8.2 | 8.9 | 8.2 |
| 8.5 | 4.2 | 6.9 | 8.1 | 9.0 | 9.8 | 9.3 |
| 9.0 | 3.8 | 6.3 | 7.4 | 8.1 | 8.7 | 8.1 |
| Temperature (oC) | ||||||
| 25 | 2.9 | 4.0 | 4.8 | 5.5 | 6.9 | 5.7 |
| 30 | 4.2 | 5.6 | 6.1 | 7.4 | 8.2 | 7.4 |
| 35 | 5.3 | 6.2 | 7.7 | 8.3 | 9.7 | 8.8 |
| 45 | 4.3 | 5.7 | 6.3 | 7.4 | 8.5 | 8.0 |
| 50 | 2.7 | 3.9 | 4.4 | 5.1 | 6.3 | 5.6 |
| 55 | 1.8 | 2.1 | 3.0 | 3.9 | 4.9 | 4.0 |
| Inoculum Size (Spores/ml) | ||||||
| 1x105 | 3.2 | 4.4 | 5.0 | 6.0 | 6.9 | 5.8 |
| 1x106 | 4.5 | 5.8 | 6.4 | 7.6 | 8.4 | 7.5 |
| 1x107 | 5.1 | 6.0 | 7.5 | 8.1 | 9.6 | 8.5 |
| 1x108 | 6.4 | 7.9 | 9.1 | 10.2 | 11.0 | 10.4 |
| 1x109 | 4.9 | 6.0 | 7.2 | 8.0 | 9.1 | 8.3 |
| Agitation speed (rev/min) | ||||||
| 140 | 3.6 | 4.7 | 5.2 | 6.0 | 7.1 | 6.1 |
| 160 | 4.7 | 5.7 | 6.8 | 7.7 | 8.5 | 7.6 |
| 180 | 6.2 | 7.1 | 8.8 | 9.2 | 10.6 | 9.5 |
| 200 | 6.8 | 7.9 | 9.7 | 11.0 | 12.1 | 11.3 |
| 220 | 6.5 | 7.4 | 8.7 | 9.4 | 11.4 | 9.8 |