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Purification profile of L-asparaginase from S.gulbargensis
| Step | Total activity (IU) | Total protein (mg) | Specific activity (IU/mg) | Purification fold | Yield (%) |
|---|---|---|---|---|---|
| Crude Extract | 3200 | 128 | 25 | 0 | 100 |
| Ammonium sulfate precipitation | 1620 | 36 | 45 | 1.8 | 50.6 |
| Sephacryl S-200 gel filtration | 1210 | 1.8 | 672.2 | 26.88 | 37.8 |
| CM Sephadex C-50 chromatography | 1026.5 | 0.5 | 2053 | 82.12 | 32 |
