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. 2010 Mar 1;41(1):91–96. doi: 10.1590/S1517-838220100001000015

Figure 1.

Figure 1

Figure 1

Figure 1

A) Representative TLC assay using KYC55 overlay (17). Lanes contain standard of N-3-oxo-octanoyl-HSL(1), extract of un-inoculated MSM (2) or un-inoculated TYEP + CaCl2 (3), and culture extracts of 07A strain grown in TYEP + CaCl2 in early (4) or late exponential growth phase (5). B) Effect of ABL (5 µg ml-1, part a) and HHL (7.5 µg ml-1, part b) on growth (closed squares) and proteolytic activity (closed circles) of P. fluorescens 07A cultivated in TYEP + CaCl2 medium at 22ºC and 150 rev min-1. The growth (open squares) and proteolytic activity (open circles) of cultures that were not added with the synthetic signal molecules are also shown. The means represent two independent experiments that were performed in duplicate. C) Effect of the addition of stationary phase bacterial extracts from P. fluorescens 07A on the specific proteolytic activity of standardized cell suspensions in TYEP + CaCl2 medium (closed circles). Cell densities were standardized at 3 x 107 CFU ml-1 (part a), 3 x 108 CFU ml-1 (part b) and 3 x 109 CFU ml-1(part c). Control cultures (open circles) were added with an extract prepared from the un-inoculated TYEP + CaCl2 medium. The means are representative of three independent experiments.