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Concentrations of variables of liquid medium in Plackett-Burman design for solid-state fermentation.
| Designation | Variable | Low level (-)Per liter | High level (+)Per liter |
|---|---|---|---|
| X1 | Peptone | 5.0g | 15.0g |
| X2 | Glucose | 80g | 160g |
| X3 | Glycerin | 16ml | 32ml |
| X4 | NaCl | 2.0g | 8.0g |
| X5 | NH4Cl | 2.0g | 8.0g |
| D1 | Dummy 1 | - | - |
| X6 | MgSO4.7H2O | 0.1g | 0.9g |
| X7 | CaCl2.2H2O | 0.0g | 0.6g |
| X8 | MnSO4.H2O | 0.0g | 1.5g |
| X9 | Malt extract | 2.0g | 8.0g |
| D2 | Dummy 2 | - | - |
