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. 2010 Sep 1;41(3):649–667. doi: 10.1590/S1517-83822010000300016

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Rhodamine 6G accumulation in Gram-positive and Gram-negative bacterial cells (panel a.) and PCR amplification of HAE1 fragment using as template the DNA extracted from Gram-positive and Gram-negative bacteria (panel b.)

Panel a. After incubation at 28°C for 24 h, bacterial colonies was observed under visible white light and UV light; Mycobacterium sp. IBB_Po1 (spots 1), Oerskovia sp. IBB_Po2 (spots 2), Corynebacterium sp. IBB_Po3 (spots 3), Chryseomonas sp. IBB_Po7 (spots 4), Pseudomonas sp. IBB_Po10 (spots 5), Burkholderia sp. IBB_Po12 (spots 6).

Panel b. 1 kb DNA ladder, Promega (lane M₁); Mycobacterium sp. IBB_Po1 (lanes 1, 2), Oerskovia sp. IBB_Po2 (lanes 3, 4), Corynebacterium sp. IBB_Po3 (lanes 5, 6), Chryseomonas sp. IBB_Po7 (lanes 7, 8), Pseudomonas sp. IBB_Po10 (lanes 9, 10), Burkholderia sp. IBB_Po12 (lanes 11, 12), negative control DNA (lanes 13, 14); anneling at 50.0°C (lanes 1, 3, 5, 7, 9, 11, 13), anneling at 52.0°C (lanes 2, 4, 6, 8, 10, 12, 14); the positions of the expected HAE1 fragment (550 bp) are indicated with arrow.