Table 1.

Phenotypic characteristics of Gram-positive and Gram-negative bacterial strains

Characteristics	Bacterial strains:			Characteristics	Bacterial strains:
	IBBPo1	IBBPo2	IBBPo3		IBBPo7	IBBPo10	IBBPo12
Gram reaction	+	+	+	Gram reaction	-	-	-
Optimal growth temperature	28°C	28°C	28°C	Optimal growth temperature	28°C	28°C	28°C
Morphology	rods	rods	rods	Morphology	rods	rods	rods
Endospore formation	-	-	-	Endospore formation	-	-	-
Mobility	-	-	-	Mobility	+	+	+
Respirator type	A	A	A	Respirator type	A	A	A
Pigment formation on:	LB	yellow	-	-	Pigment formation on:	LB	-	green	orange
	King A	-	-	-		King A	-	+	-
	King B	-	-	-		King B	-	+	-
Growth on TTC medium	-	-	-	Growth on TTC medium	-	+	-
Growth on CBB R250	-	+	-	Growth on CBB R250	+	-	-
Catalase	+	+	+	Catalase	+	+	-
Oxidase	-	-	+	Oxidase	-	+	+
Nitrates reduction	-	+	-	Nitrates reduction	+	+	+
Pyrazinamidase	+	+	+	Indole production	-	-	-
Pyrolidonyl arylamidase	+	+	+	D-glucose fermentation	-	-	+
Alkaline phosphatase	+	+	+	L-arginine dihydrolase	-	+	-
β-glucuronidase	-	-	-	Urease	-	+	+
β-galactosidase	+	-	-	Esculin hydrolysis	+	-	+
α-glucosidase	+	+	-	Gelatin hydrolysis	+	+	+
N-acetyl-β-glucosaminidase	-	+	-	β-galactosidase	-	+	+
β-glucosidase/esculin	+	+	-	D-glucose assimilation	+	+	+
Urease	-	-	-	L-arabinose assimilation	+	-	+
Gelatin hydrolysis	+	+	-	D-mannose assimilation	+	-	+
Fermentation (negative control)	-	-	-	D-mannitol assimilation	+	+	+
Glucose fermentation	+	+	-	N-acetyl-glucosamine assimilation	+	+	+
Ribose fermentation	+	+	-	D-maltose assimilation	+	-	+
Xylose fermentation	+	-	-	Potassium gluconate assimilation	+	+	+
Mannitol fermentation	+	-	-	Capric acid assimilation	+	+	+
Maltose fermentation	+	+	-	Adipic acid assimilation	-	+	+
Lactose fermentation	+	-	-	Malic acid assimilation	+	+	+
Saccharose fermentation	+	-	-	Trisodium citrate assimilation	-	+	+
Glycogen fermentation	-	+	-	Phenylacetic acid assimilation	-	-	+
G+C content (%)	71.2	70.5	68.0	G+C content (%)	51.1	66.6	66.9
MIC90 (μg ml-1)	Ampicillin	500	800	250	MIC90 (μg ml-1)	Ampicillin	1000	1000	1000
	Kanamycin	50	50	75		Kanamycin	45	550	250
	SDS	40	75	50		SDS	1000	1000	1000
	Rhodamine 6G	100	100	100		Rhodamine 6G	1000	1000	1000
PCRHAE1	A24f2-A577r2	-	-	-	PCRHAE	A24f2-A577r2	-	+	+

Legend: + = positive, - = negative, A = aerobic, MIC₉₀ = Minimum Inhibitory Concentration required to inhibit the growth of 90% of the bacteria; PCR_HAE1 = PCR amplification of HAE1 (hydrophobe/amphiphile efflux 1) fragment using as template the DNA extracted from Gram-positive and Gram-negative bacterial strains, + = expected size (550 bp) fragment was amplified, - = expected size fragment was not amplified.