Table 4.
β-galactosidase activity of Gram-positive and Gram-negative bacteria in the presence of mixture of saturated, monoaromatic and polyaromatic hydrocarbons
| Bacterial strain | Variant: | |||||||
|---|---|---|---|---|---|---|---|---|
| Control | Mixture of saturated hydrocarbon1 (5% v/v) | Mixture of monoaromatic hydrocarbons2 (0.5% v/v) | Mixture of polyaromatic hydrocarbons3 (5% v/v) | |||||
| β-Gal4 | Ind. fold | β-Gal4 | Ind. fold | β-Gal4 | Ind. fold | β-Gal4 | Ind. fold | |
| Mycobacterium sp. IBBPo1 | 10.7±0.5 | 1.0 | 39.3±0.7 | 3.7 | 42.2±0.6 | 3.9 | 17.1±0.8 | 1.6 |
| Oerskovia sp.IBBPo2 | - | - | - | - | - | - | - | - |
| Corynebacterium sp. IBBPo3 | - | - | - | - | - | - | - | - |
| Chryseomonas sp. IBBPo7 | - | - | - | - | - | - | - | - |
| Pseudomonas sp. IBBPo10 | 8.6±0.7 | 1.0 | 13.2±0.5 | 1.5 | 15.9±0.9 | 1.8 | 13.6±0.8 | 1.6 |
| Burkholderia sp. IBBPo12 | 9.7±0.6 | 1.0 | 12.2±0.7 | 1.3 | 17.6±0.6 | 1.8 | 18.7±0.9 | 1.9 |
Legend: 1= mixture of n-hexane, n-hexadecane, cyclohexane; 2= mixture of benzene, toluene, ethylbenzene; 3 = mixture of naphthalene, 2-methylnaphthalene, fluorene; 4= β-galactosidase (β-Gal) activity, expressed as the change in absorbance at 420 nm minute-1 milliliter of cells-1 optical density units at 650 nm-1, was measured in permeabilized cells and was calculate the induction fold (Ind. fold).