Table 2.
Effect of metal ions on the activity of purified xylanase from Bacillus subtilis ASH. The purified enzyme was ncubated with various metal salts at 1mm concentration for 5 min at room temperature and the xylanase activity was assayed.
| Metal ion (1 mM) | Xylanase activity (IU/ml) | Xylanase activity (% of control) |
|---|---|---|
| Control | 85.3 | 100.0 |
| ZnCl2 | 73.6 | 86.3 |
| CaCl2 | 71.6 | 84.0 |
| HgCl2 | 4.9 | 5.7 |
| NaCl | 75.5 | 88.5 |
| KCl | 71.7 | 84.1 |
| FeCl3 | 78.7 | 92.3 |
| MgCl2 | 72.1 | 84.5 |
| CoCl2 | 102.4 | 120.0 |
| CdCl2 | 73.5 | 86.2 |
| NiCl2 | 70.5 | 82.6 |
| CuCl2 | 59.6 | 69.9 |
| MnCl2 | 157.8 | 185.0 |