Table 1.
PCR markers for detection of virulence gene of E. coli
| Target gene | Nucleotide sequence (5’→3’) | Size of amplified product (bp) | References |
|---|---|---|---|
| eaeA | F: GTGGCGAATACTGGCGAGACT R: CCCCATTCTTTTTCACCGTCG |
891 | 14 |
| bfpA | F: AATGGTGCTTGCGCTTGCTGC R: GCCGCTTTATCCAACCTGGTA |
326 | 14 |
| stx1 | F: AAATCGCCATTCGTTGACTACTTCT R: CAGTCGTCACTCACTGGTTTCATCA |
370 | 14 |
| stx2 | F: TGCCATTCTGGCAACTCGCGATGCA R: GGATCTTCTCCCCACTCTGACACC |
283 | 14 |
| st | F: ATTTTTCTTTCTGTATTGTCTT R: CACCCGGTACAAGCAGGATT |
190 | 15 |
| lt | F: GGC GAC AGA TTA TAC CGT GC R: CGG TCT CTA TAT TCC CTG TT |
450 | 15 |