Table 1.
Viability and presence of Campylobacter in six-day-old chicken embryos and chicken embryos or newly hatched in 21 day-old incubation from hens inoculated by intra-esophageal with C. coli.
| Protocol analysis | ||||||
|---|---|---|---|---|---|---|
| real time PCR | ||||||
| Age of incubation | Identification | Sample | Result | Specie | CFU/g | Viability |
| 8',9’,10’,11’,12’13’,14’,16’, 17’,18’,19’,20’* | yolk | N | - | - | Viable | |
| six-day-old chicken embryos | 15’ | yolk | P | C. coli | 1.7x106 | Viable |
| 21’ | yolk | N | - | - | EM 4 days | |
| 22’ | yolk | N | - | - | EM 2 days | |
| 23’ | yolk | P | C. coli | 4.4x104 | EM 5 days | |
| 24’,25’,26’,29’* | yolk | N | -- | -- | Viable | |
| 24’,25’,26’,29’* | meconium | N | -- | -- | ||
| chicken embryos or newly hatched in 21 day-old incubation | 27’ | yolk | P | C. coli | 2.8x104 | Viable |
| 27’ | meconium | N | -- | -- | ||
| 28’ | yolk | N | -- | -- | EM 21 days | |
| 28’ | meconium | N | -- | -- | ||
| 30’ | yolk | N | -- | -- | EM 4 days | |
| 31’ | meconium | N | -- | -- | EM 19 days | |
| 31’ | yolk | N | -- | -- | ||
| 32’ | yolk | N | -- | -- | EM 3 days | |
| 33’ | yolk | N | -- | -- | Viable | |
| 33’ | meconium | P | C. coli | 4.7x104 | ||
N: Negative; P: Positive
*
Analysis individual with similar results; EM: embryo mortality