Table 2.

Campylobacter sp. in albumen and yolk from SPF eggs air chamber contaminated with C.coli after 24 hours.

		Protocol analysis
		Real time PCR
Egg	Sample	Result	Specie	CFU/g	Microbiology1 (mpcr2)
1	Albumen	N	--	--	N
1	yolk	N	--	--	N
2	Albumen	N	--	--	N
2	yolk	P	C. coli	9.6 x 106	P (C.coli)
3	Albumen	P	C. coli	1.0 x 104	P (C.coli)
3	yolk	P	C. coli	3.9 x 107	P (C.coli)
4	Albumen	P	C. coli	5.2 x 104	N
4	yolk	P	C. coli	7.9 x 106	P (C.coli)
5	Albumen	P	C. coli	1.9 x 104	P (C.coli)
5	yolk	N	--	--	N
6	Albumen	P	C. coli	2.4 x 107	P (C.coli)
6	yolk	P	C. coli	7.9 x 106	N

¹plate culture

²multiplex PCR

N: Negative; P: Positive