Table 2.
Time (hours) | Penicillin titers before extraction* (g/L) | Penicillin titers after extraction* (g/L) | ||||||
---|---|---|---|---|---|---|---|---|
UAF R1 | CSL | SCB | PA | C | CSL | SCB | PA | C |
50 | - | - | - | - | - | - | - | - |
100 | 0.48±0.01 | 1.20±0.03 | - | - | 0.72±0.02 | 1.92±0.06 | - | - |
150 | 1.08±0.03 | 1.68±0.05 | 0.48±0.03 | - | 1.20±0.05 | 1.92±0.04 | 0.72±0.03 | - |
200 | 1.08±0.02 | 1.08±0.02 | 0.72±0.04 | 0.48±0.01 | 1.20±0.03 | 1.20±0.02 | 1.08±0.02 | 0.72±0.01 |
250 | 0.72±0.04 | 1.08±0.03 | 0.72±0.01 | 0.48±0.01 | 1.08±0.02 | 1.20±0.02 | 1.08±0.04 | 0.72±0.03 |
300 | 0.48±0.01 | 0.48±0.02 | 0.48±0.01 | 0.48±0.03 | 0.72±0.02 | 0.72±0.01 | 0.72±0.02 | 0.72±0.02 |
UAF R2 | CSL | SCB | PA | C | CSL | SCB | PA | C |
50 | - | - | - | - | - | - | - | - |
100 | 0.48±0.02 | 0.72±0.02 | - | - | 0.72±0.02 | 1.08±0.03 | 0.48±0.01 | - |
150 | 0.72±0.03 | 1.68±0.06 | 0.72±0.03 | - | 1.08±0.03 | 1.92±0.06 | 1.08±0.02 | - |
200 | 1.08±0.04 | 1.20±0.04 | 0.72±0.02 | 0.48±0.01 | 1.20±0.04 | 1.44±0.04 | 1.08±0.04 | 0.72±0.01 |
250 | 1.08±0.04 | 1.08±0.03 | 0.72±0.02 | 0.48±0.02 | 1.20±0.03 | 1.20±0.03 | 1.08±0.03 | 0.72±0.02 |
300 | 0.48±0.01 | 0.48±0.01 | 0.48±0.01 | 0.48±0.02 | 0.72±0.02 | 0.72±0.02 | 0.72±0.02 | 0.72±0.03 |
CSL = Corn Steep Liquor
SCB = Sugar Cane Bagasse
PA = Phenyl Acetate
C = Control
- = Zero titers
Extraction of penicillin was carried out by 4 step solvent transfer method