Table 2.
Purification step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Yield (%) | Purification (fold) |
---|---|---|---|---|---|
Crude extract | 34.2 | 92.8 | 2.71 | 100.0 | 1.0 |
Macro-prep DEAE | 2.2 | 71.6 | 32.55 | 77.2 | 12.0 |
Sephacryl S-300 | 1.4 | 59.9 | 42.78 | 64.5 | 15.9 |