Table 1.

Microorganisms and specific primers for PCR.

Microorganism	Primer (5’-3’)	PCR annealing temperature	Extension of amplicon (bp)
A actinomycetemcomitans	F - GCT AAT ACC GCG TAG AGT CGG	50°C	557
	R - ATT TCA CAC CTC ACT TAA AGG T
P. gingivalis	F - AGG CAG CTT GCC ATA CTG CG	60° C	404
	R - ACT GTT AGC AAC TAC CGA TGT
T. forsythia	F - GCG TAT GTA ACC TGC CCG CA	60° C	641
	R - TGC TTC AGT GTC AGT TAT ACC T
T. denticola	F - TAA TAC CGA ATG TGC TCA TTT ACA T	60° C	316
	R- TCA AAG AAG CAT TCC CTC TTC TTC TTA