Table 1.

Experimental variables at different levels used for the production of protease by Bravibacterium linens using Plackett-Burman design.

Variables	Codes	Units	Experimental values
			Lower (-1.000)	Centre (0.000)	Higher (1.000)
Incubation Period	A	hrs	24	48	72
Moisture content	B	%	75	85	95
Inoculum Size	C	% (v/w)	0.1	2.55	5
Wheat bran	D	g	0	7.5	15
Soybean meal	E	g	0	7.5	15
Yeast extract	F	g	0.01	0.51	1.0
MgSO4.7H2O	G	g	0.1	0.3	0.5
(NH4)2SO4	H	g	0.01	0.51	1.0
Temperature	I	0C	30	37	44