Table 5.

Central Composite Design of the nutrients and physical parameters in real units for the response of protease along with its predicted & observed values.

Run No.	Experimental values				Protease (U/g)
	Inoculum size	Wheat bran	Soybean meal	(NH4)2SO4	Obserevd	Predicted
1	2.55	7.50	7.50	0.51	6324±224	6383
2	0.10	0.00	15.00	0.01	3367±119	3367
3	2.55	-7.50	7.50	0.51	2610±92	2569
4	7.45	7.50	7.50	0.51	3246±115	3097
5	2.55	22.50	7.50	0.51	3808±135	3735
6	0.10	0.00	0.00	0.01	1256±44	1263
7	5.00	0.00	15.00	1.00	5207±185	5188
8	2.55	7.50	-7.50	0.51	2364±175	2310
9	0.10	15.00	0.00	1.00	2430±179	2408
10	2.55	7.50	7.50	-0.48	5395±248	5382
11	5.00	15.00	15.00	0.01	6733±298	6782
12	0.10	15.00	15.00	1.00	2220±28	2237
13	5.00	0.00	0.00	1.00	1287±37	1326
14	-2.35	7.50	7.50	0.51	55±2	41.6
15	2.55	7.50	7.50	0.51	6309±185	6383
16	2.55	7.50	7.50	1.50	5981±176	6036
17	2.55	7.50	7.50	0.51	6232±183	6383
18	2.55	7.50	7.50	0.51	6504±207	6383
19	2.55	7.50	22.50	0.51	5483±175	5509
20	2.55	7.50	7.50	0.51	6268±200	6383
21	5.00	15.00	0.00	0.01	6089±194	6182