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Purification steps and yield of the endo-1,4-β glucanase activities of T. virens isolate PFHT9 from fermenting palm-fruit husk.
| Concentration method | Total protein (mg) | Total activity (nKat) | Specific activity (nKat/mg) | Yield (%) | Purification fold |
|---|---|---|---|---|---|
| Crude extract | 227.74 | 159.97 | 0.70 | 100.00 | 1.00 |
| Ammonium sulphate | 193.81 | 80.70 | 0.42 | 50.44 | 0.59 |
| Column chromatography | 147.94 | 590.31 | 3.99 | 369.03 | 6.57 |
