Table 3.

Preliminary characterization of halophilic enzymes

Sample Site	Microorganism	Potent enzyme produced	Production level (UmL-1)	pH optima	Salt optima (%, NaCl)	Temp. optima (°C)	Solvent-stability† (25%, v/v)	Thermal stability (°C) #
Sambhar Lake	Haloalkaliphilic bacterium EMB1	Protease	28	9.5	5	50	Stable	50
	Haloalkaliphilic bacterium EMB2	Protease	37	10.0	3	50	Stable	50
	Haloalkaliphilic bacterium EMB3	Protease	21	9.0	6	45	Stable	50
	Haloalkaliphilic bacterium EMB4	Amylase	1.2	9.0	3	40	Stable	50
Kozhikode	Marinobactersp. EMB5	Lipase	129	9.0	2	50	Stable	60
	Marinobactersp. EMB6	Lipase	88	9.5	2	65	Stable	60
	Virgibacillussp. EMB7	Protease	156	7.5	1	60	Stable	50
	Marinobactersp. EMB8	Amylase	4	7.0	1	45	Stable	50
Goa	Bacillussp. EMB9	Protease	191	9.0	1	60	*	*
	Oceanobacillussp. EMB10	Protease	26	9.0	1	55	*	*
Triveni Sangam	Chromohalobactersp. EMB12	Lipase	65	9.5	2	65	Stable	50
	Virgibacillussp. EMB13	Protease	62	7.5	1	60	Stable	50
	Halobacillussp. EMB14	Amylase	0.3	7.5	1	55	*	*
Nagoa	Halobacillussp. EMB15	Amylase	0.2	8.0	1	55	*	*
	Virgibacillussp. EMB16	Protease	60	7.5	1	60	*	*
	Halobacillussp. EMB17	Amylase	0.2	7.5	1	55	*	*
	Haloalkaliphilic bacterium D-10-102	Lipase	7	9.5	1.5	65	*	*
Somnath	Halomonassp. EMB11	Lipase	42	9.0	2	65	*	*
	Haloalkaliphilic bacterium EMB18	Protease	38	10.0	2	55	Stable	55
	Haloalkaliphilic bacterium S-15-9	Lipase	57	9.5	1.5	65	Stable	60
Veraval	Haloalkaliphilic bacterium Ve2-20-92	Lipase	15	9.5	1.5	65	*	*

^*Not defined

^†Solvent stability checked in hexane, cyclohexane, decane, dodecane and toluene

^#Stability for 1h