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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Mol Microbiol. 2013 Jul 29;89(5):949–962. doi: 10.1111/mmi.12323

Fig. 2. shyA and shyC are synthetically lethal.

Fig. 2

(A) TD541 (ΔshyA ΔshyC PIPTG:shyA) was grown to stationary phase in LB medium containing IPTG and spotted on LB agar plates containing either IPTG or glucose. (B) ShyA depletion in liquid culture. TD541 was grown in medium containing IPTG, washed twice in LB medium with glucose and resuspended in an equal volume LB/glucose. Cells were imaged just before addition of glucose and three hours after and analyzed using MicrobeTracker. (C) Average elongation rates of around 100 cells of wild type, TD536 (ΔshyA) and TD541 before and after ShyA depletion. Cells were grown on agarose pads with 10 % LB and glucose or IPTG and imaged every minute. Elongation rate is the slope of length increase over time. Asterisks indicate statistical significance (t-test, p<0.01) (D) Average cell widths in liquid cultures of wild type, TD536 and TD541 before and after ShyA depletion. (E) Vesicles emanating from TD541 cells grown on agarose pads contain outer membrane. A functional mCherry fusion of the outer membrane protein LpoA (Vc0581) was expressed from its native promoter and imaged 120 min after plating on an agarose pad containing glucose.